Test Categories: Blood & Marrow Transplant

Laboratory Category Test Name Description Turn Around Times Sample, Packaging & Shipping Requirements Form
HLA Blood & Marrow Transplant Buccal Swab DNA Isolation for High Resolution Testing

Isolation of highly purified DNA from a buccal swab sample for high resolution testing

NA Buccal swab HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant Buccal Swab DNA Isolation for Low/Intermediate Resolution

Isolation of highly purified DNA from a buccal swab sample for low to intermediate resolution testing

NA Buccal swab HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant CD3

Determination of absolute count & percentage of CD3 T lymphocytes in peripheral blood or apheresis product for subsequent donor leukocyte infusion by flow cytometry

3 hours from sample receipt, when lab notified in advance Samples must be received within 24 hours of collection. EDTA 3mL of anticoagulated whole blood; EDTA 0.5mL of pheresis unit, cord blood or bone marrow anticoagulated HLA Requisition Form BS 320H
CTL Blood & Marrow Transplant CD34 Enumeration with Viability

Determination of absolute count, percentage, and viability of CD34 progenitor cells in peripheral blood, pheresis product or bone marrow by flow cytometry

2 hours from sample receipt, when lab notified in advance Samples must be received within 24 hours of collection. EDTA 3mL of anticoagulated whole blood; EDTA 0.5mL of pheresis unit, cord blood or bone marrow anticoagulated CTL Requisition Form HPP 203
HLA Blood & Marrow Transplant Chimerism Studies

Testing of post transplant blood samples for percentage mixture of donor and patient DNA

3-5 Business Days Pre-transplant DNA from recipient and donor; Post-transplant sample from recipient; 5-7 mL EDTA HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA (A,B,C,DR,DQ) HR for Patient or unrelated Donor Typing to include DRB3/4/5

Sequenced Based Typing (SBT) and/or DNA based assay for HLA A, B C, DR and DQ alleles provides a high-resolution HLA phenotype for new transplant patients and their potential unrelated donors

7±2 Business Days Adults (normal WBC): 10-20 mL EDTA; Pediatrics: 3-7 mL EDTA or anticoagulated whole blood, depending on age of child; Cord blood sample: 0.5 - 1ml; Buccal swabs are acceptable HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA (A,B,C) Supplemental HR

DNA based assay provides high resolution (4 digits) typing of HLA A,B,C alleles following preliminary HLA (A,B,DR) IR typing of transplant patient siblings

5-7 Business Days Adults (normal WBC): 10-20 mL EDTA; Pediatrics: 3-7 mL EDTA or anticoagulated whole blood, depending on age of child; Buccal swabs are acceptable HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA (A,B,DR) IR

DNA based assay provides initial intermediate resolution (2 digits) typing of HLA A, B, DR for preliminary assessment of histocompatibility in siblings of transplant recipient

5-7 Business Days Adults (normal WBC): 10-20 mL EDTA; Pediatrics: 3-7 mL EDTA or anticoagulated whole blood, depending on age of child; Cord blood sample: 0.5 - 1ml; Buccal swabs are acceptable HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA (DR,DQ) Supplemental HR to include DRB3/4/5

DNA based assay provides high resolution (4 digits) typing to determine phenotype for selected siblings of transplant recipient

5-7 Business Days Adults (normal WBC): 10-20 mL EDTA; Pediatrics: 3-7 mL EDTA or anticoagulated whole blood, depending on age of child; Buccal swabs are acceptable HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA A, Supplemental HR

DNA based assay provides high resolution (4 digits) typing of HLA A alleles with previous LR results

5-7 Business Days Adults (normal WBC): 10-20 mL EDTA; Pediatrics: 3-7 mL EDTA or anticoagulated whole blood, depending on age of child; Buccal swabs are acceptable or frozen DNA HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA B, Supplemental HR

DNA based assay to provide high resolution (4 digits) typing of HLA B alleles with previous LR results

5-7 Business Days Adults (normal WBC): 10-20 mL EDTA; Pediatrics: 3-7 mL EDTA or anticoagulated whole blood, depending on age of child; Buccal swabs are acceptable or frozen DNA HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA C, Supplemental HR

DNA based assay to provide high resolution (4 digits) typing of HLA C alleles with previous LR results

5-7 Business Days Adults (normal WBC): 10-20 mL EDTA; Pediatrics: 3-7 mL EDTA or anticoagulated whole blood, depending on age of child; Buccal swabs are acceptable or frozen DNA HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA Class I (A,B,C) HR

DNA based assay used to achieve definition at high resolution (4 digits) of Class I HLA-A,B,C alleles

7±2 Business Days Adults (normal WBC): 10-20 mL EDTA; Pediatrics: 3-7 mL EDTA or anticoagulated whole blood, depending on age of child; Buccal swabs are acceptable HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA Class I (A,B,C) IR

DNA based assay provides low to intermediate resolution (2 digits) assignment of Class I HLA-A,B,C alleles

5-7 Business Days Adults (normal WBC): 10-20 mL EDTA; Pediatrics: 3-7 mL EDTA or anticoagulated whole blood, depending on age of child; Buccal swabs are acceptable HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA Class I Single Locus IR (e.g. B*27)

DNA based typing of any single locus provides intermediate resolution (2 digits) typing to determine presence or absence of 1 or 2 antigens/alleles named by client for disease association or research purposes

7-10 Business Days 5-10mL EDTA HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA Class II (DR,DQ) HR to include DRB3/4/5

DNA based assay provides high resolution (4 digits) typing of HLA DRB1, DRB3/4/5 and DQB1 alleles following preliminary HLA (A,B,DR) IR typing of transplant patient siblings

7±2 Business Days Adults (normal WBC): 10-20 mL EDTA; Pediatrics: 3-7 mL EDTA or anticoagulated whole blood, depending on age of child; Buccal swabs are acceptable HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA Class II (DR,DQ) IR

DNA based assay provides low to intermediate resolution (2 digits) assignment of Class II HLA DR, DQ alleles.

5-7 Business Days Adults (normal WBC): 10-20 mL EDTA; Pediatrics: 3-7 mL EDTA or anticoagulated whole blood, depending on age of child; Buccal swabs are acceptable HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA Class II Single Locus IR (e.g. DQ2, DQ8)

DNA based typing of any single locus provides intermediate resolution (2 digits) typing to determine presence or absence of 1 or 2 antigens/alleles named by client for disease association or research purposes

7-10 Business Days 5-10mL EDTA HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA DPA1 HR

DNA based assay to provide high resolution (4 digits) typing of HLA DPA1 alleles

5-7 Business Days Adults (normal WBC): 10-20 mL EDTA; Pediatrics: 3-7 mL EDTA or anticoagulated whole blood, depending on age of child; Buccal swabs are acceptable HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA DPB1 HR

DNA based assay to provide high resolution (4 digits) typing of HLA DPB1 alleles

5-7 Business Days Adults (normal WBC): 10-20 mL EDTA; Pediatrics: 3-7 mL EDTA or anticoagulated whole blood, depending on age of child; Buccal swabs are acceptable HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA DQA1 HR

DNA based assay to provide high resolution (4 digits) typing of HLA DQA1 alleles

5-7 Business Days Adults (normal WBC): 10-20 mL EDTA; Pediatrics: 3-7 mL EDTA or anticoagulated whole blood, depending on age of child; Buccal swabs are acceptable HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA DQB1 Supplemental HR

DNA based assay provides high resolution (4 digits) typing of HLA DQB1 alleles with previous LR results

5-7 Business Days Adults (normal WBC): 10-20 mL EDTA; Pediatrics: 3-7 mL EDTA or anticoagulated whole blood, depending on age of child; Buccal swabs are acceptable or frozen DNA HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA DRB1 Supplemental HR

DNA based assay provides high resolution (4 digits) typing of HLA DRB1 alleles with previous LR results

5-7 Business Days Adults (normal WBC): 10-20 mL EDTA; Pediatrics: 3-7 mL EDTA or anticoagulated whole blood, depending on age of child; Buccal swabs are acceptable or frozen DNA HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA DRB3/4/5 Supplemental HR

DNA based assay provides high resolution (4 digits) typing of HLA DRB3/4/5 alleles with previous LR results

5-7 Business Days Adults (normal WBC): 10-20 mL EDTA; Pediatrics: 3-7 mL EDTA or anticoagulated whole blood, depending on age of child; Buccal swabs are acceptable or frozen DNA HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA Priority Services

Reduced reporting time arranged by lab supervisor

NA NA HLA Requisition Form BS 320H
HLA Blood & Marrow Transplant HLA STAT After Hours

Reduced reporting time arranged by lab supervisor

NA NA HLA Requisition Form BS 320H