Test Categories: Platelet Refractory Panel

Laboratory Category Test Name Description Turn Around Times Sample, Packaging & Shipping Requirements Form
HLA Blood Donor Typing, Platelet Refractory Panel HLA (A,B) IR for Apheresis Platelet Donors

DNA based assay provides intermediate resolution (2 digits) of HLA-A,B alleles. Typing apheresis donor is required prior to donating for HLA matched platelets.

21-30 business days 5-7 mL EDTA; shipped within 7 days of collection HLA Requisition Form BS 320H
HLA, IRL Platelet Refractory Panel, Platelet Testing HLA (A,B) IR for Patient Platelet Transfusions

DNA based assay provides intermediate resolution (2 digits) typing of HLA-A,B alleles. Typing required for patient before search for HLA matched platelets.

48 hours Adults: 10-20 mL EDTA; Adults WBC <4x10^6/mL or 4000/µL: 20-40 mL EDTA; Pediatrics: Consult with laboratory Form BS 313, HLA Requisition Form BS 320H
HLA, IRL HLA Class I & II Antibody Screen and ID, Platelet Refractory Panel HLA Antibody ID Class I

Identification of antibody specificities to HLA Class I (A,B,C) antigens by Luminex microbead array methodology

3 business days 10 mL clotted whole blood in red top tube; blood specimens may be kept at room temperature and shipped at room temp or 24C up to 4 days after collection; OR separated serum from clot tube may be refrigerated at 4C and shipped up to 7 days from date of draw Form BS 313, HLA Requisition Form BS 320H
HLA, IRL HLA Class I & II Antibody Screen and ID, Platelet Refractory Panel HLA Antibody Screen

Screen for antibodies to HLA Class I (A,B,C) and Class II (DR,DQ,DP) and MIC antigens by Luminex microbead array methodology

3 business days 10 mL clotted whole blood in red top tube; blood specimens may be kept at room temperature and shipped at room temp or 24C up to 4 days after collection; OR separated serum from clot tube may be refrigerated at 4C and shipped up to 7 days from date of draw Form BS 313, HLA Requisition Form BS 320H
HLA, IRL Platelet Refractory Panel HLA Matched Platelet Pheresis Donor Search

Search of maintained registry of previously HLA typed donors to identify suitably HLA matched or compatible platelet pheresis donors for patients who are refractory to random donor platelets due to HLA antibodies. For patients that have previously completed HLA A,B typing or concurrent orders for HLA typing.

Same Day NA HLA Requisition Form BS 320H
HLA, IRL Platelet Refractory Panel, Platelet Testing Platelet Antibody Detection

The Capture-P Ready Screen Solid Phase System is designed to detect IgG- HPA and -HLA antibodies. The assay does NOT differentiate between HLA or HPA antibodies. The assay is not designed to detect complement components or antibodies of the IgM or IgA class.

Depends upon request; See TAT description 3-7 mL EDTA (plasma) or serum tube (no gel separators). Sample must be received in the IRL no later than 30 hrs after collection. Shipped in a container maintaining temperature between 1C - 10C Form BS 313
IRL Platelet Refractory Panel, Platelet Testing Platelet Crossmatch – SP

The Capture-P Solid Phase System is designed to detect IgG HPA and HLA antibodies.  The assay is not designed to detect complement components or antibodies of the IgM or IgA class.

Depends upon request; See TAT description 3-7 mL EDTA (plasma) or serum (not from gel separator tubes). ; Must be received within 30 hours of collection. ; Shipper to maintain temperature between 1C - 10C Form BS 313
HLA, IRL Platelet Refractory Panel Platelet Refractory Panel

Platelet antibody screen and crossmatch, HLA A,B typing, HLA matched platelet donor search, HLA antibody screen (if positive, HLA antibody identification Class I)

48 hours Three EDTA whole blood, and 5-7 mL clot (serum) tube (no gel separators). Sample must be received in the IRL no later than 30 hrs after collection. Icteric, hemolyzed and/or lipemic samples interfere with test and should not be tested as per package insert. Shipped in a container maintaining temperature between 1C - 10C Form BS 313, HLA Requisition Form BS 320H