Test Categories: Processing Tests

Laboratory Category Test Name Description Turn Around Times Sample, Packaging & Shipping Requirements Form
CTL Processing Tests Colony Forming Units (CFU)

Culture of hematopoietic progenitor cells (HPCs) including erythroid, myeloid and megakaryocytic

14 to 16 days Cryovial, product bag, cell culture medium, eg RPMI, IMDM CTL Requisition Form HPP 203
CTL Processing Tests Complete Blood Count

Total nucleated cell count, hematocrit and a breakout of mononuclear cell and granulocyte percentages

24 to 48 hours Sample size is 0.5mL, red top tube, shipped at 1-10 C CTL Requisition Form HPP 203
CTL Processing Tests Manual Differential – on product received at BSL

Manual white blood cell count differential

24 hours Cryovial, product bag, EDTA tube CTL Requisition Form HPP 203
CTL Processing Tests Sterility

Bacterial and fungal cultures on product

14 to 16 days Cryovial, product bag, tissue transport medium CTL Requisition Form HPP 203
CTL Processing Tests Trypan Blue Viability

Simple dye exclusion test to measure the percentage of viable white cells

2 hours; M-F 8am-8pm 0.5mL of anticoagulant HPC product, shipped at 1 -10 C CTL Requisition Form HPP 203