CTL |
Autologous & Allogeneic Stem Cells |
ABO Incompatibility Processing – Plasma Depletion |
Removal of plasma |
NA |
NA |
CTL |
Autologous & Allogeneic Stem Cells |
ABO Incompatibility Processing – Red Cell Depletion |
Removal of red cells from product received |
NA |
NA |
CTL |
Autologous & Allogeneic Stem Cells |
Base Lab Processing of Pheripheral Blood Progenitor Cells and Bone Marrow (Autologous & Allogeneic) |
Processing, splitting and labeling additional bags from original collection. Includes testing for CD34, CD3, Total Nucleated Count, Manual Differential, Sterility, Viability, Infectious Disease Markers and cryopreservation of up to 6 bags for storage up to 36 months. |
NA |
NA |
CTL |
Autologous & Allogeneic Stem Cells |
Base Lab Processing of Pheripheral Blood Progenitor Cells and Bone Marrow After Hours |
Base lab processing from 2300 to 0600, weekends and holidays |
NA |
NA |
CTL |
Blood & Marrow Transplant |
CD34 Enumeration with Viability |
Determination of absolute count, percentage, and viability of CD34 progenitor cells in peripheral blood, pheresis product or bone marrow by flow cytometry |
2 hours from sample receipt, when lab notified in advance |
Samples must be received within 24 hours of collection. EDTA 3mL of anticoagulated whole blood; EDTA 0.5mL of pheresis unit, cord blood or bone marrow anticoagulated |
CTL |
Processing Tests |
Colony Forming Units (CFU) |
Culture of hematopoietic progenitor cells (HPCs) including erythroid, myeloid and megakaryocytic |
14 to 16 days |
Cryovial, product bag, cell culture medium, eg RPMI, IMDM |
CTL |
Processing Tests |
Complete Blood Count |
Total nucleated cell count, hematocrit and a breakout of mononuclear cell and granulocyte percentages |
24 to 48 hours |
Sample size is 0.5mL, red top tube, shipped at 1-10 C |
CTL |
Autologous & Allogeneic Stem Cells |
Cord Blood Dilution – No Wash (with pre & post CD34) |
Dilution of DMSO from cord product with CD 34 testing |
NA |
NA |
CTL |
Autologous & Allogeneic Stem Cells |
Cord Blood Dilution – No Wash (without CD34) |
Dilution of DMSO from cord product |
NA |
NA |
CTL |
Autologous & Allogeneic Stem Cells |
Fresh Allogeneic Processing without cryopreservation, Bone Marrow or Pheripheral Blood Progenitor Cells |
Base lab processing services and testing for fresh products listed |
NA |
NA |
CTL |
Autologous & Allogeneic Stem Cells |
Labeling Extra Bags after the 6th |
Utilizing extra bags during processing for product storage, including labeling |
NA |
NA |
CTL |
Processing Tests |
Manual Differential – on product received at BSL |
Manual white blood cell count differential |
24 hours |
Cryovial, product bag, EDTA tube |
CTL |
Autologous & Allogeneic Stem Cells |
Sepax Cord Blood Product Wash (per bag) |
Automated cord blood washing in a functionally closed system |
NA |
NA |
CTL |
Processing Tests |
Sterility |
Bacterial and fungal cultures on product |
14 to 16 days |
Cryovial, product bag, tissue transport medium |
CTL |
Storage with or without Cryopreservation |
Storage of Peripheral Blood Progenitor Cells and Bone Marrow |
Stem Cell Storage per bag with no processing or cryopreservation up to 36 months |
NA |
NA |
CTL |
Storage with or without Cryopreservation |
Storage of Peripheral Blood Progenitor Cells and Bone Marrow with cryopreservation |
Cryopreservation for each bag over 6 with storage up to 36 months |
NA |
NA |
CTL |
Autologous & Allogeneic Stem Cells |
T-Cell Therapeutic Processing |
Base lab processing services and testing and up to 36 months of cryopreservation for up to 6 bags |
NA |
NA |
CTL |
Processing Tests |
Trypan Blue Viability |
Simple dye exclusion test to measure the percentage of viable white cells |
2 hours; M-F 8am-8pm |
0.5mL of anticoagulant HPC product, shipped at 1 -10 C |
CTL |
Autologous & Allogeneic Stem Cells |
Volume Reduction |
Reduce plasma as required for cryopreservation purposes |
NA |
NA |
CTL |
Autologous & Allogeneic Stem Cells |
Wash – Manual (with pre & post CD34) |
Thawing of previously frozen harvest with washing and CD34 testing |
NA |
NA |
CTL |
Autologous & Allogeneic Stem Cells |
Wash – Manual (without CD34) |
Thawing of previously frozen harvest with washing |
NA |
NA |