Cellular Therapy Laboratory

Our cellular therapy laboratory is dedicated to providing blood and marrow transplants for patients undergoing treatment for certain cancers. These products provide hematopoietic progenitor cells (HPCs), which are the precursors to red blood cells, white blood cells and platelets.

In the case of patients being infused with their own (autologous) HPCs, Vitalant handles the processing, cryopreservation and storage of the cells for transplant following collection, and then the delivery and bedside preparation when the patient is ready to be reinfused with the healthy cells. When patients require HPCs from a donor (allogeneic), Vitalant performs HLA (human leukocyte antigen) matches on relatives or on unrelated donors who are registered through the National Marrow Donor Program^®.

For the hospital oncology and transplant programs treating these patients, using Vitalant laboratories avoids the burden of obtaining complex new certifications and the expense of outside testing. The service is all-inclusive, giving clinical teams the continuity of providers while offering peace of mind for their patients that their own or their donor’s cells are treated with care and respect through every stage in their treatment process.

The cellular therapy laboratory is accredited by AABB and Foundation for the Accreditation of Cellular Therapy (FACT) and is an FDA-registered tissue bank.

For clinical consultations, please call (602) 343-7103.

View sample of the CTL Requisition Form (HPP 163).

View sample of the CTL Requisition Form (HPP 203).

IRL

(602) 343-7133
Requisition Forms

HLA

(623) 487-6400
Requisition Forms

CTL

(602) 343-7103
Requisition Forms

(602) 343-7133
Requisition Forms

Complete Test Listings

Category:

Laboratory	Category	Test Name	Description	Turn Around Times	Sample, Packaging & Shipping Requirements	Form
CTL	Autologous & Allogeneic Stem Cells	ABO Incompatibility Processing – Plasma Depletion	Removal of plasma	NA	NA	CTL Requisition Form HPP 163
CTL	Autologous & Allogeneic Stem Cells	ABO Incompatibility Processing – Red Cell Depletion	Removal of red cells from product received	NA	NA	CTL Requisition Form HPP 163
CTL	Autologous & Allogeneic Stem Cells	Base Lab Processing of Pheripheral Blood Progenitor Cells and Bone Marrow (Autologous & Allogeneic)	Processing, splitting and labeling additional bags from original collection. Includes testing for CD34, CD3, Total Nucleated Count, Manual Differential, Sterility, Viability, Infectious Disease Markers and cryopreservation of up to 6 bags for storage up to 36 months.	NA	NA	CTL Requisition Form HPP 163
CTL	Autologous & Allogeneic Stem Cells	Base Lab Processing of Pheripheral Blood Progenitor Cells and Bone Marrow After Hours	Base lab processing from 2300 to 0600, weekends and holidays	NA	NA	CTL Requisition Form HPP 163
CTL	Blood & Marrow Transplant	CD34 Enumeration with Viability	Determination of absolute count, percentage, and viability of CD34 progenitor cells in peripheral blood, pheresis product or bone marrow by flow cytometry	2 hours from sample receipt, when lab notified in advance	Samples must be received within 24 hours of collection. EDTA 3mL of anticoagulated whole blood; EDTA 0.5mL of pheresis unit, cord blood or bone marrow anticoagulated	CTL Requisition Form HPP 203
CTL	Processing Tests	Colony Forming Units (CFU)	Culture of hematopoietic progenitor cells (HPCs) including erythroid, myeloid and megakaryocytic	14 to 16 days	Cryovial, product bag, cell culture medium, eg RPMI, IMDM	CTL Requisition Form HPP 203
CTL	Processing Tests	Complete Blood Count	Total nucleated cell count, hematocrit and a breakout of mononuclear cell and granulocyte percentages	24 to 48 hours	Sample size is 0.5mL, red top tube, shipped at 1-10 C	CTL Requisition Form HPP 203
CTL	Autologous & Allogeneic Stem Cells	Cord Blood Dilution – No Wash (with pre & post CD34)	Dilution of DMSO from cord product with CD 34 testing	NA	NA	CTL Requisition Form HPP 163
CTL	Autologous & Allogeneic Stem Cells	Cord Blood Dilution – No Wash (without CD34)	Dilution of DMSO from cord product	NA	NA	CTL Requisition Form HPP 163
CTL	Autologous & Allogeneic Stem Cells	Fresh Allogeneic Processing without cryopreservation, Bone Marrow or Pheripheral Blood Progenitor Cells	Base lab processing services and testing for fresh products listed	NA	NA	CTL Requisition Form HPP 163
CTL	Autologous & Allogeneic Stem Cells	Labeling Extra Bags after the 6th	Utilizing extra bags during processing for product storage, including labeling	NA	NA	CTL Requisition Form HPP 163
CTL	Processing Tests	Manual Differential – on product received at BSL	Manual white blood cell count differential	24 hours	Cryovial, product bag, EDTA tube	CTL Requisition Form HPP 203
CTL	Autologous & Allogeneic Stem Cells	Sepax Cord Blood Product Wash (per bag)	Automated cord blood washing in a functionally closed system	NA	NA	CTL Requisition Form HPP 163
CTL	Processing Tests	Sterility	Bacterial and fungal cultures on product	14 to 16 days	Cryovial, product bag, tissue transport medium	CTL Requisition Form HPP 203
CTL	Storage with or without Cryopreservation	Storage of Peripheral Blood Progenitor Cells and Bone Marrow	Stem Cell Storage per bag with no processing or cryopreservation up to 36 months	NA	NA	CTL Requisition Form HPP 163
CTL	Storage with or without Cryopreservation	Storage of Peripheral Blood Progenitor Cells and Bone Marrow with cryopreservation	Cryopreservation for each bag over 6 with storage up to 36 months	NA	NA	CTL Requisition Form HPP 163
CTL	Autologous & Allogeneic Stem Cells	T-Cell Therapeutic Processing	Base lab processing services and testing and up to 36 months of cryopreservation for up to 6 bags	NA	NA	CTL Requisition Form HPP 163
CTL	Processing Tests	Trypan Blue Viability	Simple dye exclusion test to measure the percentage of viable white cells	2 hours; M-F 8am-8pm	0.5mL of anticoagulant HPC product, shipped at 1 -10 C	CTL Requisition Form HPP 203
CTL	Autologous & Allogeneic Stem Cells	Volume Reduction	Reduce plasma as required for cryopreservation purposes	NA	NA	CTL Requisition Form HPP 163
CTL	Autologous & Allogeneic Stem Cells	Wash – Manual (with pre & post CD34)	Thawing of previously frozen harvest with washing and CD34 testing	NA	NA	CTL Requisition Form HPP 163
CTL	Autologous & Allogeneic Stem Cells	Wash – Manual (without CD34)	Thawing of previously frozen harvest with washing	NA	NA	CTL Requisition Form HPP 163

Laboratory	CTL
Category	Autologous & Allogeneic Stem Cells
Test Name	ABO Incompatibility Processing – Plasma Depletion
Description	Removal of plasma
Turn Around Times	NA
Sample, Packaging & Shipping Requirements	NA
Form	CTL Requisition Form HPP 163

Laboratory	CTL
Category	Autologous & Allogeneic Stem Cells
Test Name	ABO Incompatibility Processing – Red Cell Depletion
Description	Removal of red cells from product received
Turn Around Times	NA
Sample, Packaging & Shipping Requirements	NA
Form	CTL Requisition Form HPP 163

Laboratory	CTL
Category	Autologous & Allogeneic Stem Cells
Test Name	Base Lab Processing of Pheripheral Blood Progenitor Cells and Bone Marrow (Autologous & Allogeneic)
Description	Processing, splitting and labeling additional bags from original collection. Includes testing for CD34, CD3, Total Nucleated Count, Manual Differential, Sterility, Viability, Infectious Disease Markers and cryopreservation of up to 6 bags for storage up to 36 months.
Turn Around Times	NA
Sample, Packaging & Shipping Requirements	NA
Form	CTL Requisition Form HPP 163

Laboratory	CTL
Category	Autologous & Allogeneic Stem Cells
Test Name	Base Lab Processing of Pheripheral Blood Progenitor Cells and Bone Marrow After Hours
Description	Base lab processing from 2300 to 0600, weekends and holidays
Turn Around Times	NA
Sample, Packaging & Shipping Requirements	NA
Form	CTL Requisition Form HPP 163

Laboratory	CTL
Category	Blood & Marrow Transplant
Test Name	CD34 Enumeration with Viability
Description	Determination of absolute count, percentage, and viability of CD34 progenitor cells in peripheral blood, pheresis product or bone marrow by flow cytometry
Turn Around Times	2 hours from sample receipt, when lab notified in advance
Sample, Packaging & Shipping Requirements	Samples must be received within 24 hours of collection. EDTA 3mL of anticoagulated whole blood; EDTA 0.5mL of pheresis unit, cord blood or bone marrow anticoagulated
Form	CTL Requisition Form HPP 203

Laboratory	CTL
Category	Processing Tests
Test Name	Colony Forming Units (CFU)
Description	Culture of hematopoietic progenitor cells (HPCs) including erythroid, myeloid and megakaryocytic
Turn Around Times	14 to 16 days
Sample, Packaging & Shipping Requirements	Cryovial, product bag, cell culture medium, eg RPMI, IMDM
Form	CTL Requisition Form HPP 203

Laboratory	CTL
Category	Processing Tests
Test Name	Complete Blood Count
Description	Total nucleated cell count, hematocrit and a breakout of mononuclear cell and granulocyte percentages
Turn Around Times	24 to 48 hours
Sample, Packaging & Shipping Requirements	Sample size is 0.5mL, red top tube, shipped at 1-10 C
Form	CTL Requisition Form HPP 203

Laboratory	CTL
Category	Autologous & Allogeneic Stem Cells
Test Name	Cord Blood Dilution – No Wash (with pre & post CD34)
Description	Dilution of DMSO from cord product with CD 34 testing
Turn Around Times	NA
Sample, Packaging & Shipping Requirements	NA
Form	CTL Requisition Form HPP 163

Laboratory	CTL
Category	Autologous & Allogeneic Stem Cells
Test Name	Cord Blood Dilution – No Wash (without CD34)
Description	Dilution of DMSO from cord product
Turn Around Times	NA
Sample, Packaging & Shipping Requirements	NA
Form	CTL Requisition Form HPP 163

Laboratory	CTL
Category	Autologous & Allogeneic Stem Cells
Test Name	Fresh Allogeneic Processing without cryopreservation, Bone Marrow or Pheripheral Blood Progenitor Cells
Description	Base lab processing services and testing for fresh products listed
Turn Around Times	NA
Sample, Packaging & Shipping Requirements	NA
Form	CTL Requisition Form HPP 163

Laboratory	CTL
Category	Autologous & Allogeneic Stem Cells
Test Name	Labeling Extra Bags after the 6th
Description	Utilizing extra bags during processing for product storage, including labeling
Turn Around Times	NA
Sample, Packaging & Shipping Requirements	NA
Form	CTL Requisition Form HPP 163

Laboratory	CTL
Category	Processing Tests
Test Name	Manual Differential – on product received at BSL
Description	Manual white blood cell count differential
Turn Around Times	24 hours
Sample, Packaging & Shipping Requirements	Cryovial, product bag, EDTA tube
Form	CTL Requisition Form HPP 203

Laboratory	CTL
Category	Autologous & Allogeneic Stem Cells
Test Name	Sepax Cord Blood Product Wash (per bag)
Description	Automated cord blood washing in a functionally closed system
Turn Around Times	NA
Sample, Packaging & Shipping Requirements	NA
Form	CTL Requisition Form HPP 163

Laboratory	CTL
Category	Processing Tests
Test Name	Sterility
Description	Bacterial and fungal cultures on product
Turn Around Times	14 to 16 days
Sample, Packaging & Shipping Requirements	Cryovial, product bag, tissue transport medium
Form	CTL Requisition Form HPP 203

Laboratory	CTL
Category	Storage with or without Cryopreservation
Test Name	Storage of Peripheral Blood Progenitor Cells and Bone Marrow
Description	Stem Cell Storage per bag with no processing or cryopreservation up to 36 months
Turn Around Times	NA
Sample, Packaging & Shipping Requirements	NA
Form	CTL Requisition Form HPP 163

Laboratory	CTL
Category	Storage with or without Cryopreservation
Test Name	Storage of Peripheral Blood Progenitor Cells and Bone Marrow with cryopreservation
Description	Cryopreservation for each bag over 6 with storage up to 36 months
Turn Around Times	NA
Sample, Packaging & Shipping Requirements	NA
Form	CTL Requisition Form HPP 163

Laboratory	CTL
Category	Autologous & Allogeneic Stem Cells
Test Name	T-Cell Therapeutic Processing
Description	Base lab processing services and testing and up to 36 months of cryopreservation for up to 6 bags
Turn Around Times	NA
Sample, Packaging & Shipping Requirements	NA
Form	CTL Requisition Form HPP 163

Laboratory	CTL
Category	Processing Tests
Test Name	Trypan Blue Viability
Description	Simple dye exclusion test to measure the percentage of viable white cells
Turn Around Times	2 hours; M-F 8am-8pm
Sample, Packaging & Shipping Requirements	0.5mL of anticoagulant HPC product, shipped at 1 -10 C
Form	CTL Requisition Form HPP 203

Laboratory	CTL
Category	Autologous & Allogeneic Stem Cells
Test Name	Volume Reduction
Description	Reduce plasma as required for cryopreservation purposes
Turn Around Times	NA
Sample, Packaging & Shipping Requirements	NA
Form	CTL Requisition Form HPP 163

Laboratory	CTL
Category	Autologous & Allogeneic Stem Cells
Test Name	Wash – Manual (with pre & post CD34)
Description	Thawing of previously frozen harvest with washing and CD34 testing
Turn Around Times	NA
Sample, Packaging & Shipping Requirements	NA
Form	CTL Requisition Form HPP 163

Laboratory	CTL
Category	Autologous & Allogeneic Stem Cells
Test Name	Wash – Manual (without CD34)
Description	Thawing of previously frozen harvest with washing
Turn Around Times	NA
Sample, Packaging & Shipping Requirements	NA
Form	CTL Requisition Form HPP 163