Cellular Therapy Laboratory

Our cellular therapy laboratory is dedicated to providing blood and marrow transplants for patients undergoing treatment for certain cancers. These products provide hematopoietic progenitor cells (HPCs), which are the precursors to red blood cells, white blood cells and platelets.

In the case of patients being infused with their own (autologous) HPCs, Vitalant handles the processing, cryopreservation and storage of the cells for transplant following collection, and then the delivery and bedside preparation when the patient is ready to be reinfused with the healthy cells. When patients require HPCs from a donor (allogeneic), Vitalant performs HLA (human leukocyte antigen) matches on relatives or on unrelated donors who are registered through the National Marrow Donor Program®.

For the hospital oncology and transplant programs treating these patients, using Vitalant laboratories avoids the burden of obtaining complex new certifications and the expense of outside testing. The service is all-inclusive, giving clinical teams the continuity of providers while offering peace of mind for their patients that their own or their donor’s cells are treated with care and respect through every stage in their treatment process.

The cellular therapy laboratory is accredited by AABB and Foundation for the Accreditation of Cellular Therapy (FACT) and is an FDA-registered tissue bank.

For clinical consultations, please call (480) 933-7416.

Contact us to discuss our cellular therapy services.

Shipping Cellular Therapy Products

Packing Configuration

IRL
HLA
CTL
TS
HPP 163

HPP 203
Laboratory Category Test Name Description Turn Around Times Sample, Packaging & Shipping Requirements Form
CTL Autologous & Allogeneic Stem Cells ABO Incompatibility Processing – Plasma Depletion Removal of plasma NA NA
CTL Autologous & Allogeneic Stem Cells ABO Incompatibility Processing – Red Cell Depletion Removal of red cells from product received NA NA
CTL Autologous & Allogeneic Stem Cells Base Lab Processing of Pheripheral Blood Progenitor Cells and Bone Marrow (Autologous & Allogeneic) Processing, splitting and labeling additional bags from original collection. Includes testing for CD34, CD3, Total Nucleated Count, Manual Differential, Sterility, Viability, Infectious Disease Markers and cryopreservation of up to 6 bags for storage up to 36 months. NA NA
CTL Autologous & Allogeneic Stem Cells Base Lab Processing of Pheripheral Blood Progenitor Cells and Bone Marrow After Hours Base lab processing from 2300 to 0600, weekends and holidays NA NA
CTL Blood & Marrow Transplant CD34 Enumeration with Viability Determination of absolute count, percentage, and viability of CD34 progenitor cells in peripheral blood, pheresis product or bone marrow by flow cytometry 2 hours from sample receipt, when lab notified in advance Samples must be received within 24 hours of collection. EDTA 3mL of anticoagulated whole blood; EDTA 0.5mL of pheresis unit, cord blood or bone marrow anticoagulated
CTL Processing Tests Colony Forming Units (CFU) Culture of hematopoietic progenitor cells (HPCs) including erythroid, myeloid and megakaryocytic 14 to 16 days Cryovial, product bag, cell culture medium, eg RPMI, IMDM
CTL Processing Tests Complete Blood Count Total nucleated cell count, hematocrit and a breakout of mononuclear cell and granulocyte percentages 24 to 48 hours Sample size is 0.5mL, red top tube, shipped at 1-10 C
CTL Autologous & Allogeneic Stem Cells Cord Blood Dilution – No Wash (with pre & post CD34) Dilution of DMSO from cord product with CD 34 testing NA NA
CTL Autologous & Allogeneic Stem Cells Cord Blood Dilution – No Wash (without CD34) Dilution of DMSO from cord product NA NA
CTL Autologous & Allogeneic Stem Cells Fresh Allogeneic Processing without cryopreservation, Bone Marrow or Pheripheral Blood Progenitor Cells Base lab processing services and testing for fresh products listed NA NA
CTL Autologous & Allogeneic Stem Cells Labeling Extra Bags after the 6th Utilizing extra bags during processing for product storage, including labeling NA NA
CTL Processing Tests Manual Differential – on product received at BSL Manual white blood cell count differential 24 hours Cryovial, product bag, EDTA tube
CTL Autologous & Allogeneic Stem Cells Sepax Cord Blood Product Wash (per bag) Automated cord blood washing in a functionally closed system NA NA
CTL Processing Tests Sterility Bacterial and fungal cultures on product 14 to 16 days Cryovial, product bag, tissue transport medium
CTL Storage with or without Cryopreservation Storage of Peripheral Blood Progenitor Cells and Bone Marrow Stem Cell Storage per bag with no processing or cryopreservation up to 36 months NA NA
CTL Storage with or without Cryopreservation Storage of Peripheral Blood Progenitor Cells and Bone Marrow with cryopreservation Cryopreservation for each bag over 6 with storage up to 36 months NA NA
CTL Autologous & Allogeneic Stem Cells T-Cell Therapeutic Processing Base lab processing services and testing and up to 36 months of cryopreservation for up to 6 bags NA NA
CTL Processing Tests Trypan Blue Viability Simple dye exclusion test to measure the percentage of viable white cells 2 hours; M-F 8am-8pm 0.5mL of anticoagulant HPC product, shipped at 1 -10 C
CTL Autologous & Allogeneic Stem Cells Volume Reduction Reduce plasma as required for cryopreservation purposes NA NA
CTL Autologous & Allogeneic Stem Cells Wash – Manual (with pre & post CD34) Thawing of previously frozen harvest with washing and CD34 testing NA NA
CTL Autologous & Allogeneic Stem Cells Wash – Manual (without CD34) Thawing of previously frozen harvest with washing NA NA
Laboratory CTL
Category Autologous & Allogeneic Stem Cells
Test Name ABO Incompatibility Processing – Plasma Depletion
Description Removal of plasma
Turn Around Times NA
Sample, Packaging & Shipping Requirements NA
Form CTL Requisition Form HPP 163
Laboratory CTL
Category Autologous & Allogeneic Stem Cells
Test Name ABO Incompatibility Processing – Red Cell Depletion
Description Removal of red cells from product received
Turn Around Times NA
Sample, Packaging & Shipping Requirements NA
Form CTL Requisition Form HPP 163
Laboratory CTL
Category Autologous & Allogeneic Stem Cells
Test Name Base Lab Processing of Pheripheral Blood Progenitor Cells and Bone Marrow (Autologous & Allogeneic)
Description Processing, splitting and labeling additional bags from original collection. Includes testing for CD34, CD3, Total Nucleated Count, Manual Differential, Sterility, Viability, Infectious Disease Markers and cryopreservation of up to 6 bags for storage up to 36 months.
Turn Around Times NA
Sample, Packaging & Shipping Requirements NA
Form CTL Requisition Form HPP 163
Laboratory CTL
Category Autologous & Allogeneic Stem Cells
Test Name Base Lab Processing of Pheripheral Blood Progenitor Cells and Bone Marrow After Hours
Description Base lab processing from 2300 to 0600, weekends and holidays
Turn Around Times NA
Sample, Packaging & Shipping Requirements NA
Form CTL Requisition Form HPP 163
Laboratory CTL
Category Blood & Marrow Transplant
Test Name CD34 Enumeration with Viability
Description Determination of absolute count, percentage, and viability of CD34 progenitor cells in peripheral blood, pheresis product or bone marrow by flow cytometry
Turn Around Times 2 hours from sample receipt, when lab notified in advance
Sample, Packaging & Shipping Requirements Samples must be received within 24 hours of collection. EDTA 3mL of anticoagulated whole blood; EDTA 0.5mL of pheresis unit, cord blood or bone marrow anticoagulated
Form CTL Requisition Form HPP 203
Laboratory CTL
Category Processing Tests
Test Name Colony Forming Units (CFU)
Description Culture of hematopoietic progenitor cells (HPCs) including erythroid, myeloid and megakaryocytic
Turn Around Times 14 to 16 days
Sample, Packaging & Shipping Requirements Cryovial, product bag, cell culture medium, eg RPMI, IMDM
Form CTL Requisition Form HPP 203
Laboratory CTL
Category Processing Tests
Test Name Complete Blood Count
Description Total nucleated cell count, hematocrit and a breakout of mononuclear cell and granulocyte percentages
Turn Around Times 24 to 48 hours
Sample, Packaging & Shipping Requirements Sample size is 0.5mL, red top tube, shipped at 1-10 C
Form CTL Requisition Form HPP 203
Laboratory CTL
Category Autologous & Allogeneic Stem Cells
Test Name Cord Blood Dilution – No Wash (with pre & post CD34)
Description Dilution of DMSO from cord product with CD 34 testing
Turn Around Times NA
Sample, Packaging & Shipping Requirements NA
Form CTL Requisition Form HPP 163
Laboratory CTL
Category Autologous & Allogeneic Stem Cells
Test Name Cord Blood Dilution – No Wash (without CD34)
Description Dilution of DMSO from cord product
Turn Around Times NA
Sample, Packaging & Shipping Requirements NA
Form CTL Requisition Form HPP 163
Laboratory CTL
Category Autologous & Allogeneic Stem Cells
Test Name Fresh Allogeneic Processing without cryopreservation, Bone Marrow or Pheripheral Blood Progenitor Cells
Description Base lab processing services and testing for fresh products listed
Turn Around Times NA
Sample, Packaging & Shipping Requirements NA
Form CTL Requisition Form HPP 163
Laboratory CTL
Category Autologous & Allogeneic Stem Cells
Test Name Labeling Extra Bags after the 6th
Description Utilizing extra bags during processing for product storage, including labeling
Turn Around Times NA
Sample, Packaging & Shipping Requirements NA
Form CTL Requisition Form HPP 163
Laboratory CTL
Category Processing Tests
Test Name Manual Differential – on product received at BSL
Description Manual white blood cell count differential
Turn Around Times 24 hours
Sample, Packaging & Shipping Requirements Cryovial, product bag, EDTA tube
Form CTL Requisition Form HPP 203
Laboratory CTL
Category Autologous & Allogeneic Stem Cells
Test Name Sepax Cord Blood Product Wash (per bag)
Description Automated cord blood washing in a functionally closed system
Turn Around Times NA
Sample, Packaging & Shipping Requirements NA
Form CTL Requisition Form HPP 163
Laboratory CTL
Category Processing Tests
Test Name Sterility
Description Bacterial and fungal cultures on product
Turn Around Times 14 to 16 days
Sample, Packaging & Shipping Requirements Cryovial, product bag, tissue transport medium
Form CTL Requisition Form HPP 203
Laboratory CTL
Category Storage with or without Cryopreservation
Test Name Storage of Peripheral Blood Progenitor Cells and Bone Marrow
Description Stem Cell Storage per bag with no processing or cryopreservation up to 36 months
Turn Around Times NA
Sample, Packaging & Shipping Requirements NA
Form CTL Requisition Form HPP 163
Laboratory CTL
Category Storage with or without Cryopreservation
Test Name Storage of Peripheral Blood Progenitor Cells and Bone Marrow with cryopreservation
Description Cryopreservation for each bag over 6 with storage up to 36 months
Turn Around Times NA
Sample, Packaging & Shipping Requirements NA
Form CTL Requisition Form HPP 163
Laboratory CTL
Category Autologous & Allogeneic Stem Cells
Test Name T-Cell Therapeutic Processing
Description Base lab processing services and testing and up to 36 months of cryopreservation for up to 6 bags
Turn Around Times NA
Sample, Packaging & Shipping Requirements NA
Form CTL Requisition Form HPP 163
Laboratory CTL
Category Processing Tests
Test Name Trypan Blue Viability
Description Simple dye exclusion test to measure the percentage of viable white cells
Turn Around Times 2 hours; M-F 8am-8pm
Sample, Packaging & Shipping Requirements 0.5mL of anticoagulant HPC product, shipped at 1 -10 C
Form CTL Requisition Form HPP 203
Laboratory CTL
Category Autologous & Allogeneic Stem Cells
Test Name Volume Reduction
Description Reduce plasma as required for cryopreservation purposes
Turn Around Times NA
Sample, Packaging & Shipping Requirements NA
Form CTL Requisition Form HPP 163
Laboratory CTL
Category Autologous & Allogeneic Stem Cells
Test Name Wash – Manual (with pre & post CD34)
Description Thawing of previously frozen harvest with washing and CD34 testing
Turn Around Times NA
Sample, Packaging & Shipping Requirements NA
Form CTL Requisition Form HPP 163
Laboratory CTL
Category Autologous & Allogeneic Stem Cells
Test Name Wash – Manual (without CD34)
Description Thawing of previously frozen harvest with washing
Turn Around Times NA
Sample, Packaging & Shipping Requirements NA
Form CTL Requisition Form HPP 163