IRL |
Red Cell Serology |
4C Antibody Identification |
Test for identification of antibodies reactive best at 4C |
Depends upon request; See TAT description |
1 plain red top and 4 EDTA tubes (No gel separator tubes); Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
IRL |
Red Cell Serology |
ABO Discrepancy (includes A2 cells & anti-A,B) |
Resolution of ABO blood group typing discrepancies |
Depends upon request; See TAT description |
1 plain red top and 4 EDTA tubes (No gel separator tubes); Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
IRL |
Red Cell Serology |
ABO Grouping |
ABO blood group typing |
Depends upon request; See TAT description |
1 plain red top and/or EDTA tube; Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
IRL |
Red Cell Serology |
Adsorption |
Autoadsorption using patient’s own cells or allogeneic adsorption using reagent red cells of known phenotype |
Depends upon request; See TAT description |
1 plain red top and 4 EDTA tubes (No gel separator tubes); Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
IRL, Transfusion Service |
Compatibility Testing & Product Services, Red Cell Serology |
Antibody Detection (screen) |
Red cell antibody screen by multiple methodologies |
Depends upon request; See TAT description |
1 plain red top and/or EDTA tube; Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313, Transfusion Services Order Form TS 003 |
IRL, Transfusion Service |
Compatibility Testing & Product Services, Red Cell Serology |
Antibody Identification Panel |
Red cell antibody identification by multiple methodologies |
Depends upon request; See TAT description |
1 plain red top and 4 EDTA tubes (No gel separator tubes); Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313, Transfusion Services Order Form TS 003 |
IRL |
Red Cell Serology |
Antibody Screen – Gel |
Red cell antibody screen by gel technology |
Depends upon request; See TAT description |
1 plain red top and/or EDTA tube; Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
IRL |
Red Cell Serology |
Antigen Typing, Donation (per antigen) |
Donor blood typing for common antigens (serological) |
Depends upon request; See TAT description |
1 plain red top and/or EDTA tube; Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
IRL |
Red Cell Serology |
Antigen Typing, Patient (per antigen) |
Patient blood typing for common antigens (serological) |
Depends upon request; See TAT description |
1 plain red top and/or EDTA tube; Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
IRL |
Red Cell Serology |
Antigen Typing, Rare-Donation (per antigen) |
Donor blood typing for rare antigens (serological) |
Depends upon request; See TAT description |
1 plain red top and/or EDTA tube; Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
IRL |
Red Cell Serology |
Antigen Typing, Rare-Patient (per antigen) |
Patient blood typing for rare antigens (serological) |
Depends upon request; See TAT description |
1 plain red top and/or EDTA tube; Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
IRL |
Red Cell Serology |
Cold Agglutinin Screen |
Test for detection of antibodies reactive best at 4C |
Depends upon request; See TAT description |
Serum/plasma collected and separated at 37 C and 1 EDTA. Please contact the IRL. |
Form BS 313 |
IRL |
Red Cell Serology |
Cold Agglutinin Titer |
To provide instructions for determining the titer of cold reactive autoantibody for cases in which cold agglutinin disease is suspected. |
Depends upon request; See TAT description |
Serum or plasma separated from freshly collected blood; sample maintained at 37C; minimum volume 2mL |
Form BS 313 |
IRL |
Red Cell Serology |
Computer Unit Search |
Computer search of the Vitalant inventory for specifically typed products |
2 hours |
NA |
Contact IRL Laboratory |
IRL |
Red Cell Serology |
Direct Antiglobulin Test (DAT) (per test) |
The direct antiglobulin test is used to demonstrate antibodies and/or complement bound to the red cells “in-vivo”. |
Depends upon request; See TAT description |
1 EDTA tube; Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
IRL |
Red Cell Serology |
Donath-Landsteiner Test |
Diagnostic test for Paroxysmal Cold Hemoglobinuria (PCH) |
Depends upon request; See TAT description |
Serum collected and separated at 37 C and 1 EDTA. Please contact the IRL. |
Form BS 313 |
IRL |
Red Cell Serology |
Drug Dependent Antibody Studies |
Tests for identification of drug dependent antibodies |
Depends upon request; See TAT description |
2 large EDTA or red top tubes; sample of drug under investigation, in the same form (tablet, solution, capsules) that the patient is receiving |
Form BS 313 |
IRL |
Red Cell Serology |
Elution |
Removal of antibody from red cells for further identification testing |
Depends upon request; See TAT description |
2 EDTA tubes; Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
IRL |
Red Cell Serology |
Enzyme Treatment Panel |
Premodification of red blood cells using proteolytic enzymes (i.e. Ficin, Papain, etc.) |
Depends upon request; See TAT description |
NA |
Form BS 313 |
IRL |
Red Cell Serology |
Extended Phenotype |
Patient typing for common blood group antigens, excluding Rh |
Depends upon request; See TAT description |
1 EDTA tube ; Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
HLA, IRL |
Platelet Refractory Panel, Platelet Testing |
HLA (A,B) IR for Patient Platelet Transfusions |
DNA based assay provides intermediate resolution (2 digits) typing of HLA-A,B alleles. Typing required for patient before search for HLA matched platelets. |
48 hours |
Adults: 10-20 mL EDTA; Adults WBC <4x10^6/mL or 4000/µL: 20-40 mL EDTA; Pediatrics: Consult with laboratory |
Form BS 313, HLA Requisition Form BS 320H |
HLA, IRL |
HLA Class I & II Antibody Screen and ID, Platelet Refractory Panel |
HLA Antibody ID Class I |
Identification of antibody specificities to HLA Class I (A,B,C) antigens by Luminex microbead array methodology |
3 business days |
10 mL clotted whole blood in red top tube; blood specimens may be kept at room temperature and shipped at room temp or 24C up to 4 days after collection; OR separated serum from clot tube may be refrigerated at 4C and shipped up to 7 days from date of draw |
Form BS 313, HLA Requisition Form BS 320H |
HLA, IRL |
HLA Class I & II Antibody Screen and ID, Platelet Refractory Panel |
HLA Antibody Screen |
Screen for antibodies to HLA Class I (A,B,C) and Class II (DR,DQ,DP) and MIC antigens by Luminex microbead array methodology |
3 business days |
10 mL clotted whole blood in red top tube; blood specimens may be kept at room temperature and shipped at room temp or 24C up to 4 days after collection; OR separated serum from clot tube may be refrigerated at 4C and shipped up to 7 days from date of draw |
Form BS 313, HLA Requisition Form BS 320H |
HLA, IRL |
Platelet Refractory Panel |
HLA Matched Platelet Pheresis Donor Search |
Search of maintained registry of previously HLA typed donors to identify suitably HLA matched or compatible platelet pheresis donors for patients who are refractory to random donor platelets due to HLA antibodies. For patients that have previously completed HLA A,B typing or concurrent orders for HLA typing. |
Same Day |
NA |
HLA Requisition Form BS 320H |
IRL |
Red Cell Serology |
Lectin Study |
Diagnostic test for polyagglutination |
Depends upon request; See TAT description |
1 EDTA tube; Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
IRL |
Blood Donor Typing, Molecular Testing (DNA Genotyping) |
Molecular Platelet Genotyping (HPA) – Donor |
Molecular determination of 11 allelic variants that determine common Human Platelet Antigens, using multiplex PCR and microarray analysis. Includes DNA extraction and purification. |
5-7 Business Days |
1 EDTA ( 7mL) tube |
Form BS 313 |
IRL |
Molecular Testing (DNA Genotyping), Platelet Testing |
Molecular Platelet Genotyping (HPA) – Patient |
Molecular determination of 11 allelic variants that determine common Human Platelet Antigens, using multiplex PCR and microarray analysis. Includes DNA extraction and purification. |
5-7 Business Days |
2 EDTA (7 mL) tubes |
Form BS 313 |
IRL |
Blood Donor Typing, Molecular Testing (DNA Genotyping) |
Molecular Red Cell Genotyping (HEA) – Donor |
Molecular determination of 38 allelic variants that determine common and rare red cell antigens plus hemoglobin S status, using multiplex PCR and microarray analysis. Includes DNA extraction and purification. |
5-7 Business Days |
Business Days 1 EDTA ( 7mL) tube |
Form BS 313 |
IRL |
Molecular Testing (DNA Genotyping) |
Molecular Red Cell Genotyping (HEA) – Patient |
Molecular determination of 38 allelic variants that determine common and rare red cell antigens plus hemoglobin S status, using multiplex PCR and microarray analysis. Includes DNA extraction and purification. |
5-7 Business Days |
2 EDTA (7 mL) tubes |
Form BS 313 |
HLA, IRL |
Platelet Refractory Panel, Platelet Testing |
Platelet Antibody Detection |
The Capture-P Ready Screen Solid Phase System is designed to detect IgG- HPA and -HLA antibodies. The assay does NOT differentiate between HLA or HPA antibodies. The assay is not designed to detect complement components or antibodies of the IgM or IgA class. |
Depends upon request; See TAT description |
3-7 mL EDTA (plasma) or serum tube (no gel separators). Sample must be received in the IRL no later than 30 hrs after collection. Shipped in a container maintaining temperature between 1C - 10C |
Form BS 313 |
IRL |
Platelet Refractory Panel, Platelet Testing |
Platelet Crossmatch – SP |
The Capture-P Solid Phase System is designed to detect IgG HPA and HLA antibodies. The assay is not designed to detect complement components or antibodies of the IgM or IgA class. |
Depends upon request; See TAT description |
3-7 mL EDTA (plasma) or serum (not from gel separator tubes). ; Must be received within 30 hours of collection. ; Shipper to maintain temperature between 1C - 10C |
Form BS 313 |
HLA, IRL |
Platelet Refractory Panel |
Platelet Refractory Panel |
Platelet antibody screen and crossmatch, HLA A,B typing, HLA matched platelet donor search, HLA antibody screen (if positive, HLA antibody identification Class I) |
48 hours |
Three EDTA whole blood, and 5-7 mL clot (serum) tube (no gel separators). Sample must be received in the IRL no later than 30 hrs after collection. Icteric, hemolyzed and/or lipemic samples interfere with test and should not be tested as per package insert. Shipped in a container maintaining temperature between 1C - 10C |
Form BS 313, HLA Requisition Form BS 320H |
IRL |
Red Cell Serology |
Red Cell Separation |
Separation of patient red cells from transfused red cells |
Depends upon request; See TAT description |
2 EDTA tubes; Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
IRL |
Red Cell Serology |
Red Cell Treatment – EGA/CHL/DTT |
Chemical premodification of red cells for testing |
Depends upon request; See TAT description |
2 EDTA tubes; Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
IRL |
Red Cell Serology |
Rh (D) Typing |
Rh (D) typing of red cells |
Depends upon request; See TAT description |
1 EDTA tubes; Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
IRL |
Red Cell Serology |
Rh Discrepancy |
Resolution of typing discrepancy |
Depends upon request; See TAT description |
2 EDTA tubes; Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
IRL |
Red Cell Serology |
Rh Phenotype |
Common Rh antigen phenotyping, excluding Rh (D) |
Depends upon request; See TAT description |
1 EDTA tube; Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
IRL |
Red Cell Serology |
Special Serum Studies (0.01 M DTT, others) |
Selective inactivation of IgM class immunoglobulin |
Depends upon request; See TAT description |
1 plain red top and 4 EDTA tubes (No gel separator tubes); Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
IRL |
Red Cell Serology |
Special Serum Studies (Neutralization/Inhibition, i.e. Le & P1 substance) |
Antibody inhibition using blood group substances |
Depends upon request; See TAT description |
1 plain red top and 4 EDTA tubes (No gel separator tubes); Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
IRL |
Red Cell Serology |
Thermal Amplitude |
Determination of optimal temperature of reactivity of cold antibodies |
24 hours |
Serum collected and separated at 37 C and 1 EDTA. Please contact the IRL. |
Form BS 313 |
IRL |
Red Cell Serology |
Titration Studies (per titration) |
Serial dilution of the sample and testing |
NA |
1 plain red top and 1 EDTA tubes (No gel separator tubes); Shipped in a container maintaining temperature between 1C - 10 C |
Form BS 313 |
IRL |
Red Cell Serology |
Turn Around Times |
STAT: 8 hours; ASAP: 24 hours; Routine: 1-2 days |
Depends upon request; See TAT description |
Insufficient sample or incomplete patient information may result in delays |
NA |